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Inhibition of Antiviral Innate Immunity by VP3 via Blocking TBK1-TRAF3 Complex Formation and IRF3 Activation

mSystems. 2021-05; 
Tingjuan Deng, Boli Hu, Xingbo Wang, Lulu Lin, Jianwei Zhou, Yuting Xu, Yan Yan, Xiaojuan Zheng, Jiyong Zhou
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Proteins, Expression, Isolation and Analysis … sc-2003; Santa Cruz Biotechnology, Santa Cruz, CA, USA) at 4°C for 4 h. For GST pulldown assay, purified GST, and GST-VP3 protein were separately mixed with purified Flag-TRAF3 protein and GST resin (L00206; GenScript, Nanjing, China) at 4°C for 4 h. After centrifugation … Get A Quote

摘要

The host innate immune system develops various strategies to antagonize virus infection, and the pathogen subverts or evades host innate immunity for self-replication. In the present study, we discovered that infectious bursal disease virus (IBDV) VP3 protein significantly inhibits MDA5-induced beta interferon (IFN-β) expression by blocking IRF3 activation. Binding domain mapping showed that the CC1 domain of VP3 and the residue lysine-155 of tumor necrosis factor receptor-associated factor 3 (TRAF3) are essential for the interaction. Furthermore, we found that the CC1 domain was required for VP3 to downregulate MDA5-mediated IFN-β production. A ubiquitination assay showed that lysine-155 of TRAF3 was the cr... More

关键词

Avibirnavirus VP3, TNF receptor-associated factor 3, innate antiviral response, ubiquitination