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A novel assay for screening WIP1 phosphatase substrates in nuclear extracts

FEBS J. 2021-05; 
Radka Storchova, Kamila Burdova, Matous Palek, René H Medema, Libor Macurek
Products/Services Used Details Operation
Gene Synthesis … from GeneTex (Irvine, CA, USA); pS473-KAP1 (clone 11G10SC, 654102) from Biolegend; His (A00186-100) from GenScript (Piscataway, NJ, USA); secondary Alexa Fluor conjugated antibodies from Thermo Fisher Scientific (Waltham, MA, USA) … Get A Quote

摘要

Upon exposure to genotoxic stress, cells activate DNA damage response (DDR) that coordinates DNA repair with a temporal arrest in the cell cycle progression. DDR is triggered by activation of ataxia telangiectasia mutated/ataxia telangiectasia and Rad3-related protein kinases that phosphorylate multiple targets including tumor suppressor protein tumor suppressor p53 (p53). In addition, DNA damage can activate parallel stress response pathways [such as mitogen-activated protein kinase p38 alpha (p38)/MAPK-activated protein kinase 2 (MK2) kinases] contributing to establishing the cell cycle arrest. Wild-type p53-induced phosphatase 1 (WIP1) controls timely inactivation of DDR and is needed for recovery from the G... More

关键词

DNA damage response, acetylation, p53, protein phosphatase