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IgV somatic mutation of human anti-SARS-CoV-2 monoclonal antibodies governs neutralization and breadth of reactivity

JCI Insight. 2021-05; 
Mayara Garcia de Mattos Barbosa, Hui Liu, Daniel Huynh, Greg Shelley, Evan T Keller, Brian T Emmer, Emily Sherman, David Ginsburg, Andrew A Kennedy, Andrew W Tai, Christiane Wobus, Carmen Mirabeli, Thomas M Lanigan, Milagros Samaniego, Wenzhao Meng, Aaron M Rosenfeld, Eline T Luning Prak, Jeffrey L Platt, Marilia Cascalho
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Catalog Antibody … 5% CO2 and cells were retrieved, resuspended at 1 x 106 cells per mL in fresh cytokine cocktail media and then transferred to wells coated with rabbit anti-Avi-tag antibody (4 µg/mL, GenScript, Cat#A00674, RRID: AB_915553) bound to S1-C … Get A Quote

摘要

Abs that neutralize SARS-CoV-2 are thought to provide the most immediate and effective treatment for those severely afflicted by this virus. Because coronavirus potentially diversifies by mutation, broadly neutralizing Abs are especially sought. Here, we report a possibly novel approach to rapid generation of potent broadly neutralizing human anti-SARS-CoV-2 Abs. We isolated SARS-CoV-2 spike protein-specific memory B cells by panning from the blood of convalescent subjects after infection with SARS-CoV-2 and sequenced and expressed Ig genes from individual B cells as human mAbs. All of 43 human mAbs generated in this way neutralized SARS-CoV-2. Eighteen of the forty-three human mAbs exhibited half-maximal inhib... More

关键词

COVID-19, Immunoglobulins, Immunology, Molecular biology