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Inhibition of USP14 influences alphaherpesvirus proliferation by degrading viral VP16 protein via ER stress-triggered selective autophagy

Autophagy. 2021-11; 
Sheng-Li Ming , Shuang Zhang , Qi Wang , Lei Zeng , Lu-Yu Zhou , Meng-Di Wang , Ying-Xian Ma , Li-Qiang Han , Kai Zhong , He-Shui Zhu , Yi-Lin Bai , Guo-Yu Yang , Jiang Wang , Bei-Bei Chu
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Catalog Antibody anti-HA (A01244-100) were from Genscript.... Protein samples were separated by SDS-PAGE (Genscript, M00660) Get A Quote

摘要

Alphaherpesvirus infection results in severe health consequences in a wide range of hosts. USPs are the largest subfamily of deubiquitinating enzymes that play critical roles in immunity and other cellular functions. To investigate the role of USPs in alphaherpesvirus replication, we assessed 13 USP inhibitors for PRV replication. Our data showed that all the tested compounds inhibited PRV replication, with the USP14 inhibitor b-AP15 exhibiting the most dramatic effect. Ablation of USP14 also influenced PRV replication, whereas replenishment of USP14 in USP14 null cells restored viral replication. Although inhibition of USP14 induced the K63-linked ubiquitination of PRV VP16 protein, its degradation was not dep... More

关键词

Alphaherpesvirus; ER stress; PRV VP16; USP14; selective autophagy