黄金城集团

至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

A gold nanoparticles-based lateral flow assay utilizing baculovirus expressed recombinant nucleocapsid and receptor binding domain proteins for serodetection of IgG and IgM against SARS-CoV-2

Biotechnol Lett. 2022-11; 
Reda Salem, Amany M Elshamy, Noha Kamel, Soha Younes, Ohoud M Marie, Fatma R Waly, Alaa A El-Kholy, Wael Elmenofy
Products/Services Used Details Operation
Mammalian Expression … After 3 washes with 300 μL of PBS-T, 100 μL of antihuman IgG HRP-conjugated secondary antibody (GenScript (Piscataway, NJ, USA), A01854) diluted 1:3000 in PBS-T + 1% casein … Get A Quote

摘要

Serological assays for SARS-CoV-2 are being utilized at an exponential rate for surveillance programs. This enterprise was designed to develop and validate a qualitative immunochromatographic test, via the Lateral Flow Assay (LFA), for detection of immunoglobulins M and G (IgM and IgG) against both nucleocapsid (N) and the receptor-binding domain (RBD) of the spike protein of SARS-CoV-2. Both targeted proteins were cloned and expressed in baculovirus expression system utilizing insect cells Sf9. The recombinant RBD and N proteins were purified and conjugated with gold nanoparticles (AuNPs) to set up the coating antigens pad. Both anti-human IgG and IgM were dispensed on nitrocellulose membrane to capture human ... More

关键词

COVID-19, Lateral flow assay, Nucleocapsid, RBD, SARS-CoV-2