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Discovery and functional assessment of a novel adipocyte population driven by intracellular Wnt/β-catenin signaling in mammals

Elife. 2022-05; 
Zhi Liu, Tian Chen, Sicheng Zhang, Tianfang Yang, Yun Gong, Hong-Wen Deng, Ding Bai, Weidong Tian, YiPing Chen
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Molecular Biology Reagents … The purified PCR products and linearized vectors were assembled using a GenBuilder Plus kit (Genscript, L00744- 10) according to the manufacturer’s instructions. The assembled … Get A Quote

摘要

Wnt/β-catenin signaling has been well established as a potent inhibitor of adipogenesis. Here, we identified a population of adipocytes that exhibit persistent activity of Wnt/β-catenin signaling, as revealed by the Tcf/Lef-GFP reporter allele, in embryonic and adult mouse fat depots, named as Wnt adipocytes. We showed that this β-catenin-mediated signaling activation in these cells is Wnt ligand- and receptor-independent but relies on AKT/mTOR pathway and is essential for cell survival. Such adipocytes are distinct from classical ones in transcriptomic and genomic signatures and can be induced from various sources of mesenchymal stromal cells including human cells. Genetic lineage-tracing and targeted cell ... More

关键词

AKT/mTOR signaling, adipocyte, beiging, cell biology, glucose homeostasis, mouse, β-catenin signaling