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A CRISPR-based nucleic acid detection method for severe fever with thrombocytopenia syndrome virus

Virus Res. 2022-02; 
Yansong Zhang, Xuanyang Bai, Jinhui Li, Jing Xie, Huan Li, Lang Yang, Peihan Li, Peng Li, Hao Dong, Qichao Chen, Xinyan Hu, Yun Wang, Tingting Jiang, Hongbin Song, Leili Jia, Lizhong Li
Products/Services Used Details Operation
Proteins, Expression, Isolation and Analysis … kit (Zhongce Biolabs, S003ZC), RNA purification magnetic beads (Beckman, A63987), a nucleic acid extraction kit (Qiagen, 52904), Cas13 nuclease (Genscript, Z03486), Cas12/13 … Get A Quote

摘要

objective: Fever with thrombocytopenia syndrome virus (SFTS) is a tick-borne infection now known to spread among humans as an aerosol, which has resulted in several outbreaks across Asia over the past decade. As mortality is substantial, it is vital to establish a rapid, on-site nucleic acid detection method for diagnosis. Here we describe such a method for SFTSV (Dabie bandavirus) based on CRISPR-Cas13a. methods: Specific recombinase-aided amplification (RAA) primers and CRISPR (cr)RNA nucleic acid detection targets were designed and synthesized for the conserved sequence of the SFTSV genome, and fluorescent CRISPR detection was used to screen for high-sensitivity crRNAs. Colloidal immunochromatography test pa... More

关键词

CRISPR, Nucleic acid detection, RAA, Severe fever with thrombocytopenia syndrome virus