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IgG Antibodies Develop to Spike but Not to the Nucleocapsid Viral Protein in Many Asymptomatic and Light COVID-19 Cases

Viruses. 2021-09; 
Maria Tutukina, Anna Kaznadzey, Maria Kireeva, Ilya Mazo
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Mammalian Expression … After 3 washes with 300 μL of PBS-T, 100 μL of antihuman IgG HRP-conjugated secondary antibody (GenScript (Piscataway, NJ, USA), A01854) diluted 1:3000 in PBS-T + 1% casein … Get A Quote

摘要

Since SARS-CoV-2 appeared in late 2019, many studies on the immune response to COVID-19 have been conducted, but the asymptomatic or light symptom cases were somewhat understudied as respective individuals often did not seek medical help. Here, we analyze the production of the IgG antibodies to viral nucleocapsid (N) protein and receptor-binding domain (RBD) of the spike protein and assess the serum neutralization capabilities in a cohort of patients with different levels of disease severity. In half of light or asymptomatic cases the antibodies to the nucleocapsid protein, which serve as the main target in many modern test systems, were not detected. They were detected in all cases of moderate or severe sympto... More

关键词

COVID-19, RBD, SARS-CoV-2, antibody detection, nucleocapsid