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A New Family of Bacteriolytic Proteins in

Front Cell Infect Microbiol. 2021-02; 
Cyril Guilhen, Wanessa C Lima, Estelle Ifrid, Xenia Crespo-Yañez, Otmane Lamrabet, Pierre Cosson
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Proteins, Expression, Isolation and Analysis … The editor and reviewers' affiliations are the latest provided on their Loop research profiles … ) in a 4–20% acrylamide gel (SurePAGE Bis-Tris, Genscript #M00655), and transferred to a … Get A Quote

摘要

Phagocytic cells ingest and destroy bacteria efficiently and in doing so ensure the defense of the human body against infections. Phagocytic amoebae represent a powerful model system to study the intracellular mechanisms ensuring destruction of ingested bacteria in phagosomes. Here, we discovered the presence of a bacteriolytic activity against in cellular extracts from The bacteriolytic activity was detected only at a very acidic pH mimicking the conditions found in phagosomes. It was also strongly decreased in extracts of KO cells that were previously described to kill inefficiently internalized bacteria, suggesting that the activity observed is involved in killing of bacteria in phagosomes. We purified... More

关键词

Bacteriolytic D. discoideum A (BadA), Dictyostelium discoideum, Klebsiella pneumoniae, bacteriolytic proteins, intracellular killing, protein purification