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Phosphate-mediated coanchoring of RBD immunogens and molecular adjuvants to alum potentiates humoral immunity against SARS-CoV-2

Sci Adv. 2021-12; 
Kristen A Rodrigues, Sergio A Rodriguez-Aponte, Neil C Dalvie, Jeong Hyun Lee, Wuhbet Abraham, Diane G Carnathan, Luis E Jimenez, Julia T Ngo, Jason Y H Chang, Zeli Zhang, Jingyou Yu, Aiquan Chang, Catherine Nakao, Benjamin Goodwin, Christopher A Naranjo, Libin Zhang, Murillo Silva, Dan H Barouch, Guido Silvestri, Shane Crotty, J Christopher Love, Darrell J Irvine
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Catalog Antibody Nunc MaxiSorp plates (Invitrogen, catalog no. 44-2404-21) were coated with a rabbit anti–his-tag antibody (GenScript, catalog no. A00174-40) at 2 μg/ml for 4 hours at 25°C in PBS and blocked with 2% BSA in PBS overnight at 4°C Get A Quote

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[Figure: see text].

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