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Ehrlichia chaffeensis TRP120 Is a Wnt Ligand Mimetic That Interacts with Wnt Receptors and Contains a Novel Repetitive Short Linear Motif That Activates Wnt Signaling

mSphere. 2021-04; 
Madison R Rogan, LaNisha L Patterson, Caitlan D Byerly, Tian Luo, Slobodan Paessler, Veljko Veljkovic, Bethany Quade, Jere W McBride
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Recombinant Antibody Expression Mouse α-His (catalog no. A00612; GenScript, Piscataway, NJ) was used for immunofluorescent labeling of purified recombinant proteins Get A Quote

摘要

expresses the TRP120 multifunctional effector, which is known to play a role in phagocytic entry, on the surface of infectious dense-cored ehrlichiae, but a cognate host receptor has not been identified. We recently reported that activates canonical Wnt signaling in monocytes to promote bacterial uptake and intracellular survival and that TRP120 was involved in this activation event. To identify the specific mechanism of pathway activation, we hypothesized that TRP120 is a Wnt signaling ligand mimetic that initiates Wnt pathway activity through direct interaction with the Wnt pathway Frizzled family of receptors. In this study, we used confocal immunofluorescence microscopy to demonstrate very strong colocaliz... More

关键词

Ehrlichia chaffeensis, SLiM, TRP120, Wnt ligand, tandem repeat