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Receptor-like kinase BAM1 facilitates early movement of the Tobacco mosaic virus

Commun Biol. 2021-04; 
Phu-Tri Tran, Vitaly Citovsky
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Proteins, Expression, Isolation and Analysis Expression of BAM1, TM-KD, and KD or of GST-MP was confirmed by western blotting using anti-Flag M2 antibody (#F3165-.2MG, Sigma) or anti-His-tag antibody (#A00174, Genscript), respectively Get A Quote

摘要

Cell-to-cell movement is an important step for initiation and spreading of virus infection in plants. This process occurs through the intercellular connections, termed plasmodesmata (PD), and is usually mediated by one or more virus-encoded movement proteins (MP) which interact with multiple cellular factors, among them protein kinases that usually have negative effects on MP function and virus movement. In this study, we report physical and functional interaction between MP of Tobacco mosaic virus (TMV), the paradigm of PD-moving proteins, and a receptor-like kinase BAM1 from Arabidopsis and its homolog from Nicotiana benthamiana. The interacting proteins accumulated in the PD regions, colocalizing with a PD m... More

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