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An insect antifreeze protein from Anatolica polita enhances the cryoprotection of Xenopus laevis eggs and embryos

J Exp Biol. 2022-02; 
Predrag Jevtić, K Wade Elliott, Shelby E Watkins, Jonathan A Sreter, Katarina Jovic, Ian B Lehner, Paul W Baures, John G Tsavalas, Daniel L Levy, Krisztina Varga
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Custom DNA/RNA Oligos … The primary antibody was mouse anti-Trx (Genscript, A00180) used at 1:250, and the secondary antibody was anti-mouse Alexa Fluor 488 (Molecular Probes, A11001) used at 1:250 … Get A Quote

摘要

Cryoprotection is of interest in many fields of research, necessitating a greater understanding of different cryoprotective agents. Antifreeze proteins have been identified that have the ability to confer cryoprotection in certain organisms. Antifreeze proteins are an evolutionary adaptation that contributes to the freeze resistance of certain fish, insects, bacteria and plants. These proteins adsorb to an ice crystal's surface and restrict its growth within a certain temperature range. We investigated the ability of an antifreeze protein from the desert beetle Anatolica polita, ApAFP752, to confer cryoprotection in the frog Xenopus laevis. Xenopus laevis eggs and embryos microinjected with ApAFP752 exhibited r... More

关键词

Xenopus laevis, Antifreeze protein, Cryoprotection, Eggs, Embryos