黄金城集团

至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

Mechanosensitive accumulation of non-muscle myosin IIB during mitosis requires its translocation activity

iScience .. 2023-08; 
Chao Wang , Jingjing Ding , Qiaodong Wei , Shoukang Du , Xiaobo Gong , Ting Gang Chew
Products/Services Used Details Operation
Proteins, Expression, Isolation and Analysis Beta-actin antibody Genscipt Cat#A00702 Protein samples were separated in a pre-cast SDS-PAGE gel (GenScript, M00657) or the b-actin antibody (Genscript, A00702; used in Figures 4B and S1D) or the GFP antibody (HuaBio, ET1604-26 Get A Quote

摘要

Non-muscle myosin II (NMII) is a force-generating mechanosensitive enzyme that responds to mechanical forces. NMIIs mechanoaccumulate at the cell cortex in response to mechanical forces. It is essential for cells to mechanically adapt to the physical environment, failure of which results in mitotic defects when dividing in confined environment. Much less is known about how NMII mechanoaccumulation is regulated during mitosis. We show that mitotic cells respond to compressive stress by promoting accumulation of active RhoA at the cell cortex as in interphase cells. RhoA mechanoresponse during mitosis activates and stabilizes NMIIB via ROCK signaling, leading to NMIIB mechanoaccumulation at the cell cortex. Using... More

关键词

Developmental biology; Mechanobiology; Molecular biology.