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Cytochalasin B-Induced Membrane Vesicles from TRAIL-Overexpressing Mesenchymal Stem Cells Induce Extrinsic Pathway of Apoptosis in Breast Cancer Mouse Model

Curr Issues Mol Biol .. 2023-01; 
Daria S Chulpanova , Tamara V Pukhalskaia , Zarema E Gilazieva , Yuliya V Filina 1, Milana N Mansurova 1, Albert A Rizvanov 1, Valeriya V Solovyeva
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Catalog Antibody For the 18S rRNA and TRAIL gene cDNAs, the PCR primers and probe were designed using the GenScript Online Real-time PCR (TaqMan) Primer Design Tool (GenScript, Piscataway, NJ, USA) and synthesized by Evrogen, Russia (Table 1)./or with anti-β-actin HRP-conjugated antibody (1:1000; #A00730, GenScript, Piscataway, NJ, USA) for 2 h at room temperature. Get A Quote
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摘要

Tumor-necrosis-factor-associated apoptosis-inducing ligand (TRAIL) is one of the most promising therapeutic cytokines that selectively induce apoptosis in tumor cells. It is known that membrane vesicles (MVs) can carry the surface markers of parental cells. Therefore, MVs are of interest as a tool for cell-free cancer therapy. In this study, membrane vesicles were isolated from TRAIL-overexpressing mesenchymal stem cells using cytochalasin B treatment (CIMVs). To evaluate the antitumor effect of CIMVs-TRAIL in vivo, a breast cancer mouse model was produced. The animals were intratumorally injected with 50 µg of native CIMVs or CIMVs-TRAIL for 12 days with an interval of two days. Then, tumor growth rate, tumor... More

关键词

breast cancer; cancer therapy; cytochalasin B; extracellular vesicles; extrinsic apoptosis pathway; mesenchymal stem cells; tumor necrosis factor-related apoptosis-inducing ligand.