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High-Level Biosynthesis of Chlorogenic Acid from Mixed Carbon Sources of Xylose and Glucose through a Rationally Refactored Pathway Network

J Agric Food Chem. 2024-02; 
Yuhui Wang, Haining Tan, Yanling Wang, Jing Liang Qin, Xinyu Zhao, Yuhan Di, Lijie Xie, Xiaojing Zhao, Ziyu Li, Guozhen Ma, Lingyan Jiang, Bin Liu, Di Huang
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Molecular Biology Reagents Then, the upstream and downstream homologous sequences coupled with the plasmid backbones and sgRNAs were assembled to construct genome-edited plasmids using a Genbuilder Cloning Kit (catalog no. L00701, GenScript Corporation, China) Get A Quote

摘要

Chlorogenic acid (CGA) has incredible potential for various pharmaceutical, nutraceutical, and agricultural applications. However, the traditional extraction approach from plants is time-consuming, further limiting its production. Herein, we design and construct the de novo biosynthesis pathway of CGA using modular coculture engineering in Escherichia coli, which is composed of MG09 and BD07 strains. To accomplish this, the phenylalanine-deficient MG09 strain was engineered to utilize xylose preferentially and to overproduce precursor caffeic acid, while the tyrosine-deficient BD07 strain was constructed to consume glucose exclusively to enhance another precursor quinic acid availability for the biosynthesis of... More

关键词

carbon sources coutilization; chlorogenic acid; modular coculture engineering; rational flux-tuning; syntrophic coculture engineering.