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The CRISPR effector Cam1 mediates membrane depolarization for phage defence

Nature. 2024-01; 
Christian F. Baca, You Yu, Jakob T. Rostøl, Puja Majumder, Dinshaw J. Patel & Luciano A. Marraffini
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GenCrispr Cas9 Genome Editing Transferred proteins were probed with THE His Tag anti-His 6 antibodies in PBST (1:4,000 dilution) (GenScript, A00186) Get A Quote

摘要

Prokaryotic type III CRISPR–Cas systems provide immunity against viruses and plasmids using CRISPR-associated Rossman fold (CARF) protein effectors1,2,3,4,5. Recognition of transcripts of these invaders with sequences that are complementary to CRISPR RNA guides leads to the production of cyclic oligoadenylate second messengers, which bind CARF domains and trigger the activity of an effector domain6,7. Whereas most effectors degrade host and invader nucleic acids, some are predicted to contain transmembrane helices without an enzymatic function. Whether and how these CARF–transmembrane helix fusion proteins facilitate the type III CRISPR–Cas immune response remains unknown. Here we investigate the role of ... More

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