黄金城集团

至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

Role of UPF1-LIN28A interaction during early differentiation of pluripotent stem cells

Nat Commun. 2024-01; 
Seungwon Jung , Seung Hwan Ko , Narae Ahn , Jinsam Lee , Chang-Hwan Park , Jungwook Hwang
Products/Services Used Details Operation
Polyclonal Antibody Services … during early differentiation of pluripotent stem cells | Nature Communications … 2.nature communications … the following primary antibodies: anti-FLAG (GenScript, A00187; 1:2000), UPF1 (… Get A Quote

摘要

UPF1 and LIN28A are RNA-binding proteins involved in post-transcriptional regulation and stem cell differentiation. Most studies on UPF1 and LIN28A have focused on the molecular mechanisms of differentiated cells and stem cell differentiation, respectively. We reveal that LIN28A directly interacts with UPF1 before UPF1-UPF2 complexing, thereby reducing UPF1 phosphorylation and inhibiting nonsense-mediated mRNA decay (NMD). We identify the interacting domains of UPF1 and LIN28A; moreover, we develop a peptide that impairs UPF1-LIN28A interaction and augments NMD efficiency. Transcriptome analysis of human pluripotent stem cells (hPSCs) confirms that the levels of NMD targets are significantly regulated by both U... More

关键词