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Expression and purification of the mitochondrial transmembrane protein FAM210A in Escherichia coli

Protein Expr Purif. 2023-06; 
Jared Hollinger, Jiangbin Wu, Kamel M Awayda, Mitchell R O'Connell, Peng Yao
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Proteins, Expression, Isolation and Analysis … The cleavage reaction using TEV protease (Genscript, Cat. #Z03030) was subsequently … -His tag and the His-tagged TEV protease (Genscript, Cat. #Z03030) while allowing the purified … Get A Quote

摘要

The protein Family with sequence similarity 210 member A (FAM210A) is a mitochondrial inner membrane protein that regulates the protein synthesis of mitochondrial DNA encoded genes. However, how it functions in this process is not well understood. Developing and optimizing a protein purification strategy will facilitate biochemical and structural studies of FAM210A. Here, we developed a method to purify human FAM210A with deleted mitochondrial targeting signal sequence using the MBP-His fusion in Escherichia coli. The recombinant FAM210A protein was inserted into the E. coli cell membrane and purified from isolated bacterial cell membranes, followed by a two-step process using Ni-NTA resin-based immobilized-met... More

关键词

FAM210A, Mitochondria, Protein synthesis, Translation, Transmembrane