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Haemolysins are essential to the pathogenicity of deep-sea Vibrio fluvialis

iScience . 2024-03; 
Yujian Wang, Jingchang Luo, Yan Zhao, Jian Zhang, Xiaolu Guan, Li Sun
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Molecular Biology Reagents The proteins were separated in 12% SurePAGE gels (M00667, GenScript) and stained with Coomassie blue (Figure S7). The control protein SUMO were previously purified in our lab. Get A Quote

摘要

Vibrio fluvialis is an emerging foodborne pathogen that produces VFH (Vibrio fluvialis hemolysin) and δVFH (delta-Vibrio fluvialis hemolysin). The function of δVFH is unclear. Currently, no pathogenic V. fluvialis from deep sea has been reported. In this work, a deep-sea V. fluvialis isolate (V13) was examined for pathogenicity. V13 was most closely related to V. fluvialis ATCC 33809, a human isolate, but possessed 262 unique genes. V13 caused lethal infection in fish and induced pyroptosis involving activation of the NLRP3 inflammasome, caspase 1 (Casp1), and gasdermin D (GSDMD). V13 defective in VFH or VFH plus δVFH exhibited significantly weakened cytotoxicity. Recombinant δVFH induced NLRP3-Casp1-GSDMD-... More

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