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The Transpeptidase Sortase A Binds Nucleic Acids and Mediates Mammalian Cell Labeling

Adv Sci (Weinh). 2024-04; 
Yingzheng Liu, Zhike Lu, Panfeng Wu, Zhaohui Liang, Zhenxing Yu, Ke Ni, Lijia Ma
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Proteins, Expression, Isolation and Analysis … The mixture was then separated by 4–20% Bis-Tris PAGE (GenScript M00656, M00657) and transferred onto a nitrocellulose membrane (Merck HATF00010). The membranes were … Get A Quote

摘要

Wild-type sortase A is an important virulence factor displaying a diverse array of proteins on the surface of bacteria. This protein display relies on the transpeptidase activity of sortase A, which is widely engineered to allow protein ligation and protein engineering based on the interaction between sortase A and peptides. Here an unknown interaction is found between sortase A from Staphylococcus aureus and nucleic acids, in which exogenously expressed engineered sortase A binds oligonucleotides in vitro and is independent of its canonical transpeptidase activity. When incubated with mammalian cells, engineered sortase A further mediates oligonucleotide labeling to the cell surface, where sortase A attaches i... More

关键词

GAG, cell labeling by nucleic acids, multiplexed scRNA‐seq, sortase