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Identification of an FMNL2 Interactome by Quantitative Mass Spectrometry

Int J Mol Sci. 2024-05; 
Sarah Fox, Antoine Gaudreau-LaPierre, Ryan Reshke, Irina Podinic, Derrick J Gibbings, Laura Trinkle-Mulcahy, John W Copeland
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Proteins, Expression, Isolation and Analysis … Lysates were cleared by centrifugation (10 min, 16,000× g) and the supernatant was incubated with anti-DYKDDDDK agarose beads (GenScript, L00432) or GFP-trap beads (… Get A Quote

摘要

Formin Homology Proteins (Formins) are a highly conserved family of cytoskeletal regulatory proteins that participate in a diverse range of cellular processes. FMNL2 is a member of the Diaphanous-Related Formin sub-group, and previous reports suggest FMNL2's role in filopodia assembly, force generation at lamellipodia, subcellular trafficking, cell-cell junction assembly, and focal adhesion formation. How FMNL2 is recruited to these sites of action is not well understood. To shed light on how FMNL2 activity is partitioned between subcellular locations, we used biotin proximity labeling and proteomic analysis to identify an FMNL2 interactome. The interactome identified known and new FMNL2 interacting proteins wi... More

关键词

BioID, FMNL2, extracellular vesicles, formins, interactome