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OsWRKY78 regulates panicle exsertion via gibberellin signaling pathway in rice

J Integr Plant Biol. 2024-03; 
Enyang Mei, Mingliang He, Min Xu, Jiaqi Tang, Jiali Liu, Yingxiang Liu, Zhipeng Hong, Xiufeng Li, Zhenyu Wang, Qingjie Guan, Xiaojie Tian, Qingyun Bu
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Proteins, Expression, Isolation and Analysis … μL Protein-A/G magbeads (GenScript, L00277) were incubated with 2 μL anti-Flag (Abmart, M20008) for 3 h with gentle rotation at 4C; subsequently, extracts of equal total proteins were … Get A Quote

摘要

Panicle exsertion is one of the crucial agronomic traits in rice (Oryza sativa). Shortening of panicle exsertion often leads to panicle enclosure and severely reduces seed production. Gibberellin (GA) plays important roles in regulating panicle exsertion. However, the underlying mechanism and the relative regulatory network remain elusive. Here, we characterized the oswrky78 mutant showing severe panicle enclosure, and found that the defect of oswrky78 is caused by decreased bioactive GA contents. Biochemical analysis demonstrates that OsWRKY78 can directly activate GA biosynthesis and indirectly suppress GA metabolism. Moreover, we found OsWRKY78 can interact with and be phosphorylated by mitogen-activated pro... More

关键词

MAPK cascades, OsWRKY78, gibberellin, panicle exsertion, rice