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Cell death induced by nicotine in human neuroblastoma SH-SY5Y cells is mainly attributed to cytoplasmic vacuolation originating from the trans-Golgi network

Food Chem Toxicol. 2024-01; 
Jun Li, Hongjuan Wang, Huan Chen, Xiao Li, Yong Liu, Hongwei Hou, Qingyuan Hu
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Proteins, Expression, Isolation and Analysis … In total, 20 μg of total proteins were separated using 12% SurePAGE (M00669, GenScript, Nanjing, China), and transferred to polyvinylidene fluoride (PVDF) membranes. The … Get A Quote

摘要

Humans are usually exposed to nicotine through the use of tobacco products. Although it is generally believed that nicotine is relatively harmless in tobacco consumption, it is, in fact, a toxic substance that warrants careful consideration of its potential toxicity. However, the current understanding of the neurotoxicity of nicotine is still very limited. In this study, we aim to reveal the toxic risk of nicotine to key target neuronal cells and its potential toxic mechanisms. The results showed that nicotine induced cell death, ROS increase, mitochondrial membrane potential decrease, and DNA damage in SH-SY5Y human neuroblastoma cells at millimolar concentrations, but did not cause toxic effects at the physio... More

关键词

Cell death, Cell vacuolation, Neurotoxicity, Nicotine, trans-Golgi network