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DNA polymerase iota promotes EMT and metastasis of esophageal squamous cell carcinoma by interacting with USP7 to stabilize HIF-1α

Cell Death Dis. 2024-02; 
Aidi Gao, Mingxia Zhang, Shuang Qi Zhu, Shitao Zou, Hengrui Chen, Xiaoqin Li, Chao He, Liangsu Zhou, Yan Mei, Weiqun Ding, Jundong Zhou, Yue Zhou, Yuandong Cao
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Proteins, Expression, Isolation and Analysis … GST-tagged Pol ι from BL21 was immobilized on glutathione sepharose beads (GenScript, L00206). GST or GST fusion proteins were mixed with MYC-USP7 and HA-HIF-1α expressed … Get A Quote

摘要

Esophageal squamous cell carcinoma (ESCC) is one of the most lethal cancer types, with a low 5-year survival rate of ~20%. Our prior research has suggested that DNA Polymerase iota (Pol ι), a member of Y-family DNA polymerase, plays a crucial role in the invasion and metastasis of ESCC. However, the underlying mechanism is not well understood. In this study, we utilized ChIP-PCR and luciferase reporter assays to investigate the binding of HIF-1α to the promoter of the Pol ι gene. Transwell, wound healing, and mouse models were employed to assess the impact of Pol ι and HIF-1α on the motility of ESCC cells. Co-immunoprecipitation and Western blot were carried out to explore the interaction between Pol ι an... More

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