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Tafluprost promotes axon regeneration after optic nerve crush via Zn-mTOR pathway

Neuropharmacology. 2023-10; 
Siting Wu, Canying Liu, Jiahui Tang, Caiqing Wu, Qi Zhang, Zhe Liu, Jiaxu Han, Jingfei Xue, Jicheng Lin, Yuze Chen, Jinpeng Yang, Yehong Zhuo, Yiqing Li
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Proteins, Expression, Isolation and Analysis … 20 μg samples from each protein sample were loaded into 12% precast gels (M00669, GenScript, Nanjing, China) and transferred to polyvinylidene fluoride (PVDF) membranes. The … Get A Quote

摘要

objective: To investigate whether Tafluprost could promote optic nerve regeneration in mice after optic nerve crush (ONC) and determine the underlying molecular mechanism. methods: Tafluprost was injected into the vitreous body immediately after ONC. The level of Zn in the inner plexiform layer (IPL) of the retina was stained using autometallography (AMG). The number of survival retinal ganglion cells (RGCs) was determined via dual staining with RGC markers Tuj1 and RBPMS. Individual axons that regenerated to 0.25, 0.5, 0.75 and 1 mm were manually counted in the whole-mount optic nerve labeled by cholera toxin B fragment (CTB). Immunofluorescence and Western blot were performed to detect protein expression lev... More

关键词

Axonal regeneration, Optic nerve injury, Tafluprost, Zinc, mTOR