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Protein disulfide isomerase is essential for spermatogenesis in mice

JCI Insight. 2024-06; 
Yaqiong Zhang, Aizhen Yang, Zhenzhen Zhao, Fengwu Chen, Xiaofeng Yan, Yue Han, Depei Wu, Yi Wu
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Proteins, Expression, Isolation and Analysis … For immunoprecipitation, the antibody was added into testes lysates and incubated overnight at 4C, followed by incubation with protein G beads (Genscript, L00209) for 2 hours. The … Get A Quote

摘要

Spermatogenesis requires precise posttranslational control in the endoplasmic reticulum (ER), but the mechanism remains largely unknown. The protein disulfide isomerase (PDI) family is a group of thiol oxidoreductases responsible for catalyzing the disulfide bond formation of nascent proteins. In this study, we generated 14 strains of KO mice lacking the PDI family enzymes and found that only PDI deficiency caused spermatogenesis defects. Both inducible whole-body PDI-KO (UBC-Cre/Pdifl/fl) mice and premeiotic PDI-KO (Stra8-Cre/Pdifl/fl) mice experienced a significant decrease in germ cells, testicular atrophy, oligospermia, and complete male infertility. Stra8-Cre/Pdifl/fl spermatocytes had significantly upregu... More

关键词

Cell biology, Protein misfolding, Reproductive biochemistry, Reproductive biology