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Nudel/NudE and Lis1 promote dynein and dynactin interaction in the context of spindle morphogenesis.

Mol Biol Cell.. 2013-09;  24(22):3522-3533
Wang S, Ketcham SA, Schön A, Goodman B, Wang Y, Yates J 3rd, Freire E, Schroer TA, Zheng Y. Department of Embryology, Carnegie Institution for Science, Baltimore, MD 21218.
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摘要

Lis1, Nudel/NudE, and dynactin are regulators of cytoplasmic dynein, a minus end-directed, microtubule-based motor required for proper spindle assembly and orientation. In vitro studies have shown that dynactin promotes processive movement of dynein on microtubules whereas Lis1 causes dynein to enter a persistent force-generating state (referred to here as dynein stall). Yet, how the activities of Lis1, Nudel/NudE, and dynactin are coordinated to regulate dynein remains poorly understood in vivo. Working in Xenopus egg extracts, we show that Nudel/NudE facilitates the binding of Lis1 to dynein, which enhances the recruitment of dynactin to dynein. We further report a novel Lis1-dependent dynein-dynactin interac... More

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