| Products/Services Used | Details | Operation |
|---|---|---|
| Catalog Antibody | For immunoblots, the membranes were probed with the following primary antibodies, which were used according to the manufacturer’s instructions: anti-GFP (SAB43011338, Sigma, Germany), anti-mCherry (PA534974, Thermo Fisher, USA), anti-TTOP (residues 158–449; the antigen for the immunization of rabbits was bacterially-produced, GenScript China Inc.), anti-HA tag (A02040, Abbkine, China), anti-Myc tag (A02060, Abbkine, China), antiFLAG tag (A02010, Abbkine, China), anti-His tag (A02050, Abbkine, China), anti-cFBPase (AS04043, Agrisera, Sweden), and anti-actin (A01050, Abbkine, China). | Get A Quote |
In plants, thousands of nucleus-encoded proteins translated in the cytosol are sorted to chloroplasts and mitochondria by binding to specific receptors of the TOC (translocon on the outer chloroplast membrane) and the TOM (translocon on the outer mitochondrial membrane) complexes for import into those organelles. The degradation pathways for these receptors are unclear. Here, we discovered a converged ubiquitin-proteasome pathway for the degradation of Arabidopsis thaliana TOC and TOM tail-anchored receptors. The receptors are ubiquitinated by E3 ligase(s) and pulled from the outer membranes by the AAA+ adenosine triphosphatase CDC48, after which a previously uncharacterized cytosolic protein, transmembrane dom... More
