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eEF1Bγ is a positive regulator of NF-κB signaling pathway.

Biochem Biophys Res Commun.. 2014-03; 
D Liu, C Sheng, S Gao, W Jiang, J Li, C Yao, H Chen, Wu J, Chen S, Huang W. CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
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摘要

Mitochondrial antiviral-signaling protein (MAVS), as a critical adaptor of RIG-I signaling, bridges viral RNA recognition and downstream signal activation. However, the regulating mechanisms of MAVS are not well understood. In this study, we demonstrated that eukaryotic elongation factor 1B gamma (eEF1Bγ) activates NF-κB signaling pathway through targeting MAVS. GST-pull down and mass spectrometric analysis suggested that eEF1Bγ binds to the CARD domain of MAVS. The interaction and mitochondrial colocalization of eEF1Bγ and MAVS were further verified by co-immunoprecipitation (co-IP) and immunofluorescence microscopy assays. The dual-luciferase assays showed that ectopic expression of eE... More

关键词

NF-κB signaling pathway; Mitochondrial antiviral-signaling protein (MAVS); Eukaryotic elongation factor 1B gamma (eEF1Bγ); Ubiquitination