Figure 1.
Purification of DYKDDDDK‐tagged protein from E. coli lysate

Lanes:
1. Whole cell lysate
2. Flow through
3. Protein marker
4‐8. Eluate of target DYKDDDDK‐tagged protein (M.W. 60.4 kDa)

Figure 2.
Purification of N‐ terminus and Cterminus DYKDDDDK ‐tagged proteins

Lanes:
1‐2．Eluate of N‐terminus DYKDDDDKtagged protein (M.W. 19.2 kDa)
3. Protein marker
4‐5. Eluate of C‐terminus DYKDDDDKtagged protein (M.W. 26.4 kDa)

Figure 3.
Purification of DYKDDDDK‐tagged protein from mammalian cells

Lanes:
1. Protein marker
2. Supernatant from cultured CHO cells
3. Flow through after binding
4. Eluate of target DYKDDDDK ‐tagged protein (M.W. 27.6 kDa)

Figure 4.
Purification of DYKDDDDK‐tagged glycoprotein from mammalian cells

The upper figure shows SDS‐PAGE analysis result and the lower figure shows Western blot result.

Lanes:
1. Protein marker
2. Supernatant from CHO cells expressing target glycoprotein
3. Flow through after binding
4. Eluate of target DYKDDDDK‐tagged protein (M.W. 50.0 kDa)

Figure 5.
Purification of DYKDDDDK‐tagged proteins using different elution methods.

Lanes:
1. Protein #1 (M.W. 16.8 kDa) by competitive elution using 300 μg/ml DYKDDDDK peptide.
2. Protein #2 (M.W. 54.6 kDa) by neutral elution with 3M NaCl
3. Protein #3 (M.W. 27.6 kDa) by acid elution, pH 3.5
4. Protein #1 (M.W. 16.8 kDa) by alkaline elution, pH 10.5
5. Protein #1 (M.W. 16.8 kDa) by alkaline elution, pH 12

Figure 6.
Benchmark with Sigma‐Aldrich’s ANTI‐FLAG M2 Affinity Gel

Side‐by‐side comparison of DYKDDDDKtagged protein purification with GenScript’s Anti‐DYKDDDDK G1 Affinity Resin and Sigma‐Aldrich’s ANTI‐FLAG M2 Affinity Gel, from same staring material following manufacturer suggested procedures.

Lanes:
1. Protein marker
2. Purification from M2 gel (pH 3.5 elution)
3. Purification from G1 resin (pH 12.0 elution)

Anti-DYKDDDDK G1 Affinity Resin

GenScript Anti‐DYKDDDDK G1 Affinity Resin (Cat. No. L00432) is designed for the purification of DYKDDDDK‐tagged protein from commonly used protein expression systems including bacteria, yeast and mammalian cells.

产品编号	L00432
规格	1 ml Resin (Total 2 ml) 5 ml Resin (Total 10 ml) 10 ml Resin (Total 20 ml) 25 ml Resin (Total 50 ml) 大单询价
数量
价格	￥2810


联系黄金城集团

产品概述

Product Description

DYKDDDDK or FLAG® tag is an octapeptide tag that can be added to a protein using recombinant DNA technology. It can be fused to the N‐terminus or C‐terminus of a target protein to facilitate detection and purification. GenScript Anti‐DYKDDDDK G1 Affinity Resin (Cat. No. L00432) is designed for the purification of DYKDDDDK‐tagged protein from commonly used protein expression systems including bacteria, yeast and mammalian cells. The DYKDDDDK‐tagged protein in the cell lysate can specifically bind to the anti‐DYKDDDDK monoclonal antibodies coupled to the resin. Nonspecific bindings are eliminated by stringent washing steps, and the target protein is finally eluted with high recovery rate.

Key Features

Comparison between and

		Anti-DYKDDDDK G1 Affinity Resin (L00432)	Anti-DYKDDDDK IP Resin (L00425)
Immunoprecipitation (IP)		√	√
Purification		√	n/a
Elution	Alkaline elution	√	n/a
	Alkalescent elution	√	n/a
	Acid elution	√	n/a
	Neutral elution	√	n/a
	Competitive elution	√	n/a
	SDS-PAGE loading synthesize buffer	√	√
Regeneration		4-10 times	not recommend
Binding volume		large scale	mini-volume (<1ml)

产品属性

Product Content	50% settled resin in TBS with 0.02% sodium azide
Matrix	4% cross‐linked agarose
Average Bead size	μm
Ligand	Mouse monoclonal antibody against DYKDDDDK tag, clone G1
Binding Capacity	Approximately 1.5 mg DYKDDDDK‐tagged protein per ml settled resin
Storage & Stability	Store at 2‐8 °C for up to 12 months. Do not freeze the resin.
Resin Reuse	The resin can be recycled for at least 4 times. If maintained properly, the resin can be reused 10 times with minimum loss of binding capability.
Elution Method	Acid, alkaline, neutral, peptide competitive or SDS‐PAGE loading buffer elutions* (*If elute with SDS-PAGE loading buffer, the heavy chain of G1 antibody will be denatured and release from the resin.)
Reagents Compatibility	Compatible with commonly used reagents at certain concentrations