黄金城集团

至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

Structure of a TOC-TIC supercomplex spanning two chloroplast envelope membranes

Cell. 2022-11; 
Zeyu Jin , Li Wan , Yuqi Zhang , Xuecheng Li , Yong Cao , Haobin Liu , Shengyao Fan , Du Cao , Zhengmao Wang , Xiaobo Li , Junmin Pan , Meng-Qiu Dong , Jianping Wu , Zhen Yan
Products/Services Used Details Operation
Proteins, Expression, Isolation and Analysis After centrifugation at 20,000 g for 1 h to remove the insoluble content, the supernatant was loaded onto Anti-FLAG G1 Affinity Resin (GenScript). Anti-FLAG G1 Affinity Resin ,GenScript ,Cat# L00432 Get A Quote

摘要

The TOC and TIC complexes are essential translocons that facilitate the import of the nuclear genome-encoded preproteins across the two envelope membranes of chloroplast, but their exact molecular identities and assembly remain unclear. Here, we report a cryoelectron microscopy structure of TOC-TIC supercomplex from Chlamydomonas, containing a total of 14 identified components. The preprotein-conducting pore of TOC is a hybrid β-barrel co-assembled by Toc120 and Toc75, while the potential translocation path of TIC is formed by transmembrane helices from Tic20 and YlmG, rather than a classic model of Tic110. A rigid intermembrane space (IMS) scaffold bridges two chloroplast membranes, and a large hydrophilic cl... More

关键词

Chlamydomonas reinhardtii; TOC-TIC supercomplex; chloroplast; cryo-EM structure; protein import; translocon.