Fig. A-1 (left figure): Inclusion body preparation and denaturation
Lane 1: Total protein after sonication
Lane 2: The supernatant after centrifugation
Lane 3: Washed with 1 M urea
Lane 4: Dissolved by 8 M urea
Fig. A-2 (right figure): Refolded protein
Lane 1: Refolded tag-free A protein
Fig. B-1 (left figure): Inclusion body preparation and denaturation
Lane 1: The supernatant after centrifugation
Lane 2: The precipitation after centrifugation
Lane 3: Inclusion bodies dissolved by urea
Lane 4: Flow through
Lane 5: Washed by imidazole containing urea
Lane 6: Eluted by imidazole containing urea
Fig. B-2 (right figure): Refolded protein
Lane 1 and 2: Refolded B fusion protein
-Dr. Barry Bradford
Kansas State University, USA
"GenScript provides fast, professional protein synthesis services at very reasonable prices. By making it cost-effective to outsource protein production, GenScript has made it possible for my lab to focus on our own area of expertise and get more research done. The detailed planning, updates, and reports that GenScript provides all of the quality control that one could ask for. I strongly recommend GenScript's protein production service."
