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Immunization of Pigs by DNA Prime and Recombinant Vaccinia Virus Boost To Identify and Rank African Swine Fever Virus Immunogenic and Protective Proteins.

J. Virol.. 2018-01; 
JancovichJames K,ChapmanDave,HansenDebra T,RobidaMark D,LoskutovAndrey,CraciunescuFelicia,BorovkovAlex,KiblerKaren,GoatleyLynnette,KingKatherine,NethertonChristopher L,TaylorGeraldine,JacobsBertram,SykesKathryn,DixonLin
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… antibody overnight at 37°C with shaking at 1000 rpm (Eppendorf Thermomixer) in a 142 combined 30 µL of Buffer A and 30 µL of anti-thrioredoxin mouse IgG2a monoclonal 143 antibody (GenScript cat. no. A00180). The supernatant was removed and verified to contain 144 …

摘要

African swine fever virus (ASFV) causes an acute hemorrhagic fever in domestic pigs, with high socioeconomic impact. No vaccine is available, limiting options for control. Although live attenuated ASFV can induce up to 100% protection against lethal challenge, little is known of the antigens which induce this protective response. To identify additional ASFV immunogenic and potentially protective antigens, we cloned 47 viral genes in individual plasmids for gene vaccination and in recombinant vaccinia viruses. These antigens were selected to include proteins with different functions and timing of expression. Pools of up to 22 antigens were delivered by DNA prime and recombinant vaccinia virus boost to gr... More

关键词

African swine fever virus,DNA vaccines,immune markers,vaccinia virus,veterinary vaccine develop