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Noncanonical function of DGCR8 controls mESC exit from pluripotency.

J. Cell Biol.. 2017; 
Cirera-Salinas Daniel,Yu Jian,Bodak Maxime,Ngondo Richard P,Herbert Kristina M,Ciaudo Const
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摘要

Mouse embryonic stem cells (mESCs) deficient for DGCR8, a key component of the microprocessor complex, present strong differentiation defects. However, the exact reasons impairing their commitment remain elusive. The analysis of newly generated mutant mESCs revealed that DGCR8 is essential for the exit from the pluripotency state. To dissociate canonical versus noncanonical functions of DGCR8, we complemented the mutant mESCs with a phosphomutant DGCR8, which restored microRNA levels but did not rescue the exit from pluripotency defect. Integration of omics data and RNA immunoprecipitation experiments established DGCR8 as a direct interactor of Tcf7l1 mRNA, a core component of the pluripotency netwo... More

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