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Lotus japonicus clathrin heavy Chain1 is associated with Rho-Like GTPase ROP6 and involved in nodule formation.

Plant Physiol.. 2015; 
Wang Chao,Zhu Maosheng,Duan Liujiang,Yu Haixiang,Chang Xiaojun,Li Li,Kang Heng,Feng Yong,Zhu Hui,Hong Zonglie,Zhang Zhong
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Proteins, Expression, Isolation and Analysis The supernatant (600 mL) was first subjected to preclearing by incubation for 30 min at 4°C with gentle rotation with 30 mL of protein A/G magnetic beads (L00277; GenScript), which were prewashed three times with washing buffer containing 50 mM HEPES, pH 7. Get A Quote

摘要

Mechanisms underlying nodulation factor signaling downstream of the nodulation factor receptors (NFRs) have not been fully characterized. In this study, clathrin heavy chain1 (CHC1) was shown to interact with the Rho-Like GTPase ROP6, an interaction partner of NFR5 in Lotus japonicus. The CHC1 gene was found to be expressed constitutively in all plant tissues and induced in Mesorhizobium loti-infected root hairs and nodule primordia. When expressed in leaves of Nicotiana benthamiana, CHC1 and ROP6 were colocalized at the cell circumference and within cytoplasmic punctate structures. In M. loti-infected root hairs, the CHC protein was detected in cytoplasmic punctate structures near the infection pocket ... More

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