黄金城集团

至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

Quantitative Lipid Imaging Reveals a New Signaling Function of Phosphatidylinositol-3,4-Bisphophate: Isoform- and Site-Specific Activation of Akt.

Mol Cell. 2018; 
Liu SL, Wang ZG, Hu Y, Xin Y, Singaram I, Gorai S, Zhou X, Shim Y, Min JH, Gong LW, Hay N, Zhang J, Cho W.
Products/Services Used Details Operation
Codon Optimization , 2014 Ni-NTA agarose QIAGEN 30230 Glutathione resin GenScript L00206 Experimental Models: Cell Lines Mouse NIH 3T3 ATCC CRL_1658 HEK293 ATCC CRL_1573 HeLa ATCC CCL-2 Pten / MEF (Nogueira et al.... coli BL21 RIL codon plus (Stratagene) cells and purified using the glutathione resin (GenScript). Get A Quote

摘要

Activation of class I phosphatidylinositol 3-kinase (PI3K) leads to formation of phosphatidylinositol-3,4,5-trisphophate (PIP3) and phosphatidylinositol-3,4-bisphophate (PI34P2), which spatiotemporally coordinate and regulate a myriad of cellular processes. By simultaneous quantitative imaging of PIP3 and PI34P2 in live cells, we here show that they have a distinctively different spatiotemporal distribution and history in response to growth factor stimulation, which allows them to selectively induce the membrane recruitment and activation of Akt isoforms. PI34P2 selectively activates Akt2 at both the plasma membrane and early endosomes, whereas PIP3 selectively stimulates Akt1 and Akt3 exclusively at the plasma... More

关键词

Akt activation mechanism; PI34P2 quantification; PI3K signaling; PIP3 quantification