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Development of Optimized Strategies for Growth Factor Incorporation onto Electrospun Fibrous Scaffolds To Promote Prolonged Release.

ACS Appl Mater Interfaces. 2020; 
Karpov TE, Peltek OO, Muslimov AR,, Tarakanchikova YV, Grunina TM, Poponova MS, Karyagina AS,, Chernozem RV, Pariy IO, Mukhortova YR, Zhukov MV, Surmeneva MA, Zyuzin MV, Timin AS,,, Surmenev RA.
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Proteins, Expression, Isolation and Analysis … culture medium42 The specific activity of BMP-2 used in experiments was 0109 U/μg and is similar to that of the commercially available E coli-produced BMP-2 from GenScript (Cat No Z02913), USA (0103 U/μg) Page 10 of 51 ACS Paragon Plus Environment … Get A Quote

摘要

Growth factor incorporation in biomedical constructs for their local delivery enables specific pharmacological effects such as the induction of cell growth and differentiation. This has enabled a promising way to improve the tissue regeneration process. However, it remains challenging to identify an appropriate approach that provides effective growth factor loading into biomedical constructs with their following release kinetics in a prolonged manner. In the present work, we performed a systematic study, which explores the optimal strategy of growth factor incorporation into sub-micrometric-sized CaCO3 core-shell particles (CSPs) and hollow silica particles (SiPs). These carriers were immobilized onto the surfa... More

关键词

3D polymer scaffolds; cell behavior; core−shell particles; growth factors; hollow silica particles; osteogenic differentiation; reduced graphene oxide; tissue regeneration