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Recombinant Proteins> | Gels were blotted on transfer membranes (Immobilon-P, Cat #IPVH00010), blocked with 4% BSA in TBST, and probed with the following primary antibodies: anti-RIPK1 (Novus Biologicals, NBP1-45841), anti-RIP3 (phospho S232) (Abcam, ab195117), antiMLKL (phosphor S345) (Abcam, ab196436). HRP-conjugated antibodies used include goat anti-rabbit IgG (HL)-HRP conjugate (Bio Rad, 170-6515) and THE beta actin antibody (GenScript, A00730). | Get A Quote |
Necrotizing enterocolitis (NEC) is a devastating disease of premature infants characterized by Toll-like receptor 4 (TLR4)-dependent intestinal inflammation and enterocyte death. Given that necroptosis is a proinflammatory cell death process that is linked to bacterial signaling, we investigated its potential role in NEC, and the mechanisms involved.,Human and mouse NEC intestine were analyzed for necroptosis gene expression (ie, RIPK1, RIPK3, and MLKL), and protein activation (phosphorylated RIPK3). To evaluate a potential role for necroptosis in NEC, the effects of genetic (ie, Ripk3 knockout or Mlkl knockout) or pharmacologic (ie, Nec1s) inhibition of intestinal inflammation were assessed in a mouse NEC mode... More
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