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Optimization of oncogene expression through intra-population competition.

Biotechnol J.. 2013-07; 
Ferreira JP, Wang CL. Department of Chemical Engineering, Stanford University, CA, USA.
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摘要

Although functional roles have been assigned to many genes - e.g., those involved in cell-cycle regulation, growth signaling, or cancer - considerably less is known about the quantitative relationship between their expression level and outcome. We devised an intra-population competition to study oncogene dosage. Cell populations were engineered to express a range of H-Ras oncogene levels. Cells with different levels of H-Ras then "competed" for an increased share of the total cell population. By using flow cytometry to track the population composition over time, we determined the relationship between different H-Ras oncogene expression levels and net proliferation rate. Under culture conditions where ... More

关键词

Ras oncogene; cell line engineering; gene expression optimization; inter-species competition; population dynamics