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Inhibition of MAN2A1 Enhances the Immune Response to Anti–PD-L1 in Human Tumors

Clin Cancer Res. 2020-11; 
Sailing Shi # , Shengqing Gu # , Tong Han , Wubing Zhang , Lei Huang , Ziyi Li , Deng Pan , Jingxin Fu , Jun Ge , Myles Brown , Peng Zhang , Peng Jiang , Kai W Wucherpfennig , X Shirley Liu
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Nucleic Acid Purification & Analysis For Western blotting, equal amounts of protein were heat denatured in the presence of a reducing agent and separated on 4%–12% or 10% Bis-Tris SurePAGE gels (GenScript, #M00652, #M00665), a Get A Quote

摘要

Purpose: Immune checkpoint blockade has shown remarkable efficacy, but in only a minority of patients with cancer, suggesting the need to develop additional treatment strategies. Aberrant glycosylation in tumors, resulting from the dysregulated expression of key enzymes in glycan biosynthesis, modulates the immune response. However, the role of glycan biosynthesis enzymes in antitumor immunity is poorly understood. We aimed to study the immunomodulatory effects of these enzymes. Experimental design: We integrated transcriptional profiles of treatment-naïve human tumors and functional CRISPR screens to identify glycometabolism genes with immunomodulatory effects. We further validated our findings using in vi... More

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