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Molecular cloning and functional characterization of Physcomitrella patens UBC13-UEV1 genes required for Lys63-linked polyubiquitination

Plant Sci. 2020; 
Huiping Guo, Linxiao Wang, Ruoyang Hu, Yikun He, Wei Xiao
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Catalog Antibody … analysis. The blots were probed with anti-His 6 (MBL, D291-3) and anti-GST (GenScript, A00865-100) primary antibodies, and chemiluminescence signals were detected by autoradiography. 2.6. Ub conjugation reaction. In vitro … Get A Quote

摘要

Ubc13 and Ubc/E2 variant (Uev) form a stable heterodimer to mediate Lys63-linked polyubiquitination. Unicellular eukaryotic genomes often contain single UBC13 and UEV gene; however, multiple homologs were found in higher plants. As initial land plants, Physcomitrella patens occupies a key evolutionary position between green algae and higher plants. In this study, we report the identification and functional characterization of two UBC13 and three UEV1 genes from P. patens. Both PpUbc13s form heterodimers with PpUev1B or PpUev1C, which catalyze Lys63-linked polyubiquitination in vitro and functionally complement the yeast ubc13 mms2 null mutant from killing by DNA-damaging agents. In contrast, PpUev1A is unable t... More

关键词

DNA-damage tolerance, K63-linked polyubiquitination, Physcomitrella patens, Ubc13, Uev1