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Ethylene-induced NbMYB4L is involved in resistance against tobacco mosaic virus in Nicotiana benthamiana

Mol Plant Pathol. 2021-10; 
Tong Zhu, Xue Zhou, Jian-Long Zhang, Wei-Hao Zhang, Li-Pei Zhang, Chun-Xiang You, Paula E Jameson, Peng-Tao Ma, Shan-Li Guo
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Proteins, Expression, Isolation and Analysis … Reactive proteins were then labelled with antibodies against the GFP protein (Genscript; cat. no. A01704; 1:5000), and against HA (Genscript, cat. no. A01244, 1:10,000). Actin was used as the internal control and labelled with anti- β- actin antibody (Genscript, cat. no. A00702, 1… Get A Quote

摘要

Several MYB transcription factors are known to play important roles in plant resistance to environmental stressors. However, the mechanism governing the involvement of MYBs in regulating tobacco mosaic virus (TMV) resistance in plants is still unclear. In this study, we found that not only is Nicotiana benthamiana MYB4-like involved in defence against TMV, but also that the ethylene pathway participates in MYB4L-mediated resistance. Transcription of NbMYB4L was up-regulated in N. benthamiana infected with TMV. Silencing of NbMYB4L led to intensified TMV replication, whereas overexpression of NbMYB4L induced significant resistance to TMV. Transcription of NbMYB4L was greater in 1-aminocyclopropanecarboxylic aci... More

关键词

Ethylene insensitive 3-like 1, Nicotiana benthamiana, V-myb avian myeloblastosis viral oncogene homolog 4-like, ethylene, tobacco mosaic virus, transgenic