stimulated with HK-Sp (multiplicity of infection = 10) with 71 recombinant mouse IFN-γ (rmIFN-γ; GenScript×107/mouse) and injected (i.v.) with 50 ng of 77 rmIFN-γ (GenScript) in 100 μL of PBS and injected (i.t.) with 2 μg of rmIFN-γ 78 (GenScript) in 25 μL of PBS containing 1% mouse serum or only 1% mouse mouse IFN-γ GenScript Cat#Z02916-500 Chemicals Todd–Hewitt broth BD Cat#249240 Neopeptone BD