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Tissue-specific mitotic bookmarking by hematopoietic transcription factor GATA1.

Cell.. 2012-08;  150(4):725-37
Kadauke S, Udugama MI, Pawlicki JM, Achtman JC, Jain DP, Cheng Y, Hardison RC, Blobel GA. Division of Hematology, The Children's Hospital of Philadelphia, Philadelphia, PA 19104, USA.
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...Antibodies used for western blotting are anti-CSR-1 (Claycomb et al., 2009), anti-GFP (A01704, <b>Genscript</b>), and anti-&alpha;-Tubulin (MCA78A, Serotec) antibodies...

摘要

SummaryTissue-specific transcription patterns are preserved throughout cell divisions to maintain lineage fidelity. We investigated whether transcription factor GATA1 plays a role in transmitting hematopoietic gene expression programs through mitosis when transcription is transiently silenced. Live-cell imaging revealed that a fraction of GATA1 is retained focally within mitotic chromatin. ChIP-seq of highly purified mitotic cells uncovered that key hematopoietic regulatory genes are occupied by GATA1 in mitosis. The GATA1 coregulators FOG1 and TAL1 dissociate from mitotic chromatin, suggesting that GATA1 functions as platform for their postmitotic recruitment. Mitotic GATA1 target genes tend to reactivate... More

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