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Wheat yellow mosaic virus NIb targets TaVTC2 to elicit broad-spectrum pathogen resistance in wheat.

Plant Biotechnology Journal. 2023-01; 
Zhang Tianye, Hu Haichao, Wang Ziqiong, Feng Tianyou, Yu Lu, Jie Zhang, Gao Wenqing, Zhou Yilin, Meihao Sun, Peng Liu, Zhong Kaili, Chen ZhiHui, Jianping Chen, Wei Li, Jian Yang
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Proteins, Expression, Isolation and Analysis For the immunoblot, proteins were separated on 10% SDS-PAGE gels (SurePAGE™, Genscript, M00652) through electrophoresis, and then transferred to NC membranes using eBlot™ L1 (Genscript, L00686C). Get A Quote

摘要

GDP-L-galactose phosphorylase (VTC2) catalyzes the conversion of GDP-L-galactose to L-galactose-1-P, a vital step of ascorbic acid (AsA) biosynthesis in plants. AsA is well known for its function in the amelioration of oxidative stress caused by most pathogen infection, but its function against viral infection remains unclear. Here, we have identified a VTC2 gene in wheat named as TaVTC2 and investigated its function in association with the wheat yellow mosaic virus (WYMV) infection. Our results showed that overexpression of TaVTC2 significantly increased viral accumulation, whereas knocking down TaVTC2 inhibited the viral infection in wheat, suggesting a positive regulation on viral infection by TaVTC2. Moreov... More

关键词

NIb; TaVTC2; ascorbate acid; reactive oxygen species; wheat; wheat yellow mosaic virus.