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Repeated Omicron exposures override ancestral SARS-CoV-2 immune imprinting

Nature. 2024-01; 
Ayijiang Yisimayi, Weiliang Song, Jing Wang, Fanchong Jian, Yuanling Yu, Xiaosu Chen, Yanli Xu, Sijie Yang, Xiao Niu, Tianhe Xiao, Jing Wang , Lijuan Zhao, Haiyan Sun, Ran An, Na Zhang, Yao Wang, Peng Wang, Lingling Yu, Zhe Lv, Qingqing Gu, Fei Shao, Ronghua Jin, Zhongyang Shen, Xiaoliang Sunney Xie, Youchun Wang, Yunlong Cao
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Gene Synthesis For expression optimization in human cells, heavy and light chain genes were synthesized by GenScript, inserted separately into plasmids (pCMV3-CH, pCMV3-CL or pCMV3-CK) via infusion (Vazyme, C112), and co-transfected into Expi293F cells (Thermo Fisher, A14527) using polyethylenimine transfection...After centrifugation, supernatants containing the monoclonal antibodies were purified using protein A magnetic beads (Genscript, L00695). Get A Quote

摘要

The continuing emergence of SARS-CoV-2 variants highlights the need to update COVID-19 vaccine compositions. However, immune imprinting induced by vaccination based on the ancestral (hereafter referred to as WT) strain would compromise the antibody response to Omicron-based boosters1-5. Vaccination strategies to counter immune imprinting are critically needed. Here we investigated the degree and dynamics of immune imprinting in mouse models and human cohorts, especially focusing on the role of repeated Omicron stimulation. In mice, the efficacy of single Omicron boosting is heavily limited when using variants that are antigenically distinct from WT-such as the XBB variant-and this concerning situation could be ... More

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