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Full-length versus truncated α-factor secretory signal sequences for expression of recombinant human insulin precursor in yeast Pichia pastoris: a comparison

J Genet Eng Biotechnol. 2023-05; 
Nuruliawaty Utami, Dini Nurdiani, Hariyatun Hariyatun, Eko Wahyu Putro, Fadillah Putri Patria, Wien Kusharyoto
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Proteins, Expression, Isolation and Analysis … of GenScript SurePAGE, Bis–Tris, 10 cm × 8 cm gels gradient (4–20%) M00657 (GenScript Biotech, Nanjing, Jiangsu, China) in GenScript running buffer Tris-MOPS-SDS 1 × M00138 (… Get A Quote

摘要

background: Human insulin was the first FDA-approved biopharmaceutical drug produced through recombinant DNA technology. The previous studies successfully expressed recombinant human insulin precursors (HIP) in Pichia pastoris truncated and full-length α-factor recombinant clones. The matting α-factor (Matα), a signal secretion, direct the HIP protein into the culture media. This study aimed to compare the HIP expression from full-length and truncated α-factor secretory signals clones that grown in two types of media, buffered methanol complex medium (BMMY) and methanol basal salt medium (BSMM). results: ImageJ analysis of the HIP's SDS-PAGE shows that the average HIP expression level of the recombinant P. ... More

关键词

BMMY, BSMM, Full-length α-factor, Human insulin precursor, Pichia pastoris, Secretory signal, Truncated α-factor