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A Quantitative Rabbit Model of Vaccinia Keratitis.

Invest Ophthalmol Vis Sci.. 2010-09; 
Sharon Altmann, Andrew Emanuel, Megan Toomey, Kim McIntyre, Jill Covert, Richard Redd Dubielzig, Gary Leatherberry, Christopher J. Murphy, Shantha Kodihalli, and Curtis R. Brandt. Department of Medical Microbiology and ImmunologyUniversity of Wisconsin-Madison, Madison, WI 53706, USA.
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摘要

PURPOSE: The goal of this study was to use multiple quantitative disease measures to evaluate the effect of various viral inocula on the development of vaccinia keratitis in rabbits. METHODS: Trephined eyes of female rabbits were infected with 10(4), 10(5), 10(6), or 10(7) plaque-forming units (pfu) of the Dryvax strain of the vaccinia virus and scored daily for disease for 14 days according to a modification of the MacDonald-Shadduck scoring system. Ocular viral titers and vaccinia-specific antibody titers were determined by plaque assay and ELISA, respectively. RESULTS: The amount of virus used for infection affected the severity of disease, with 10(4) pfu eliciting milder keratitis after delayed onset com... More

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