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High temperature delays and low temperature accelerates evolution of a new protein phenotype

Nat Commun. 2024-03; 
Jia Zheng, Ning Guo, Yuxiang Huang, Xiang Guo, Andreas Wagner
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Molecular Biology Reagents … , protein engineering, and biochemical assays, we determined that this is due to the protein-… -tag ELISA Detection Kit (L00436, Genscript) rather than a GFP ELISA Kit to quantify soluble … Get A Quote

摘要

Since the origin of life, temperatures on earth have fluctuated both on short and long time scales. How such changes affect the rate at which Darwinian evolution can bring forth new phenotypes remains unclear. On the one hand, high temperature may accelerate phenotypic evolution because it accelerates most biological processes. On the other hand, it may slow phenotypic evolution, because proteins are usually less stable at high temperatures and therefore less evolvable. Here, to test these hypotheses experimentally, we evolved a green fluorescent protein in E. coli towards the new phenotype of yellow fluorescence at different temperatures. Yellow fluorescence evolved most slowly at high temperature and most ra... More

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